Enzyme Experiments :: Free Essays

Enzyme Experiments Central Experiment The Action Of An Enzyme, e.g., amylase converting amylum to diminution sugar. 1. Substrate starch - use the same volume of the same starch solution each time 2. Enzyme amylase - use the same volume of the same amylase solution each time 3. further use starch and amylase solutions that test negative for reducing sugar with Benedicts Reagent 4. Four test tubes individually one third amply of (a) starch solution, (b) amylase solution, (c) starch solution and (d) water 5. Warm all to 37C in a heated water bath at 37C 6. Mix (a) and (b) - the try out. Mix (c) and (d) - the control 7. Use a buffer to maintain the pH at a constant qualified value pH 8 8. Every minute test a small sample of each for starch using iodine 9. take in Results always blue-black - starch always present, starch not miserable down 10. Experiment Result eventually a yellow-brown illusion - st arch is not present, it has been broken down 11. Now test the experiment solution for reducing sugar using Benedicts Reagent. Result brick-red colour organize - reducing sugar is now present 12. Repeat the entire function many times to verify the results 13. Conclusion amylase converts starch to reducing sugar. Only when amylase is present does starch disappear and reducing sugar appear subscriber line Fehlings can be used to test for reducing sugar. Experiment To regard the Effect of Enzyme Concentration on the Rate of Enzyme Action. Same as the primordial experiment, at different amylase concentrations, as far as 10. Make up different dilutions of the amylase solution by adding increasing amounts of water.